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MOUSE IgG2a isotype Control
Monoclonal M2A IgG2a , Unconjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Description | MOUSE IgG2a isotype control |
|---|---|
| Conjugate | Unconjugated |
| Clonality | Monoclonal |
Application 
| FC |
| Application Note | FlowCytometry 1:1000-1:5000 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG2a |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Species of Origin | Mouse |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA. |
| Storage Condition | Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
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