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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Sheep IgM    

Sheep IgM

  •  - Sheep IgM  ASR2576
    SDS-Page of Sheep IgM Whole Molecule. Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size-Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.
Product Information
Description SHEEP IgM whole molecule
Conjugate Unconjugated
Physical State Liquid (sterile filtered)
Host Isotype IgM
Buffer 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Species of Origin Sheep
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Sheep IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Sheep IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and anti-Sheep IgM ( µ chain specific).   No reaction was observed against anti-Sheep IgG F(c).  Some light chain cross reactivity will occur with anti-Sheep IgG.
Storage Condition Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.

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$ 192.00
Cat# ASR2576
(40 western blots)
Availability: 3-5 days
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