Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)
|Description||Anti-CHICKEN IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated|
|Conjugate||Alkaline Phosphatase (Calf Intestine)|
|Application ||WB, IHC, E|
|Application Note||ELISA 1:2,000-1:10,000|
Western Blot 1:500-1:2,500
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG (H&L)|
|Buffer||0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Immunogen||Chicken IgY / IgG whole molecule|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.1% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Anti-Chicken IgY / IgG Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY / IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgY / IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.|
|Storage Condition||Store Secondary Antibody at 4° C before opening. DO NOT FREEZE. This antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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