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Anti-Human IgG F(c) (Alkaline Phosphatase Conjugated) Secondary Antibody

Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)

  • DB - Anti-Human IgG F(c)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR2700
    Dot Blot of Human IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG Fc Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng Primary antibody: n/a Secondary antibody: Human IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.
Product Information
Description Anti-HUMAN IgG F(c) (RABBIT) Antibody Alkaline Phosphatase Conjugated
Host Rabbit
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Human
Reactivity Human
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG F(c)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum.  No reaction was observed against Human IgG F(ab’)2.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Human IgG F(c) antibody generated in rabbit detects specifically Anti-Human IgG F(c) fragment. This secondary antibody anti-Human IgG F(c) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 203.00
Cat# ASR2700
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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