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>   home   >   Products   >   Secondary Antibodies   >   R-Phycoerythrin (RPE)   >   Anti-Mouse IgG2b (Alkaline Phosphatase Conjugated) Pre-adsorbed Secondary Antibody   

Anti-Mouse IgG2b (Alkaline Phosphatase Conjugated) Pre-adsorbed Secondary Antibody

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

  • DB - Anti-Mouse IgG2b  (Alkaline Phosphatase Conjugated) Pre-adsorbed Secondary Antibody ASR2722
    Dot Blot of Mouse IgG2b Antibody Alkaline Phosphatase Conjugated Pre-absorbed. Antigen: Mouse IgG2b. Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng. Primary antibody: n/a. Secondary antibody: Mouse IgG2b Antibody Alkaline Phosphatase Conjugated Pre-absorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.
Product Information
Description Anti-MOUSE IgG2b (Gamma 2b chain) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins)
Host Goat
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,000
Immunochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG2b
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Mouse IgG2b heavy chain
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Mouse IgG2b alkaline phosphatase conjugated secondary antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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$ 203.00
Cat# ASR2722
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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