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>   home   >   Products   >   Secondary Antibodies   >   Texas Red®   >   Anti-Rat IgG F(ab')2 (Peroxidase Conjugated) Secondary Antibody   

Anti-Rat IgG F(ab')2 (Peroxidase Conjugated) Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

  • WB - Anti-Rat IgG F(ab')2  (Peroxidase Conjugated) Secondary Antibody ASR2798
    Western Blot of Anti-Rat IgG F(ab')2 (RABBIT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(ab')2 (RABBIT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product Information
Description Anti-RAT IgG F(ab')2 (RABBIT) Antibody Peroxidase Conjugated
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Rat
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:10,000-1:50,000
Western Blot 1:1,000-1:10,000
Immunohistochemistry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG F(ab')2
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgG F(ab')2 fragment
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum.  No reaction was observed against Rat IgG F(c).
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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$ 209.00
Cat# ASR2798
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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