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F(ab')2 Anti-Rat IgG (H&L) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, Unconjugated

     
  • WB - F(ab')2  Anti-Rat IgG (H&L)  Pre-Adsorbed Secondary Antibody ASR2818
    Western Blot of Goat anti-Rat IgG Antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat antibody at 1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rat IgG. Other band(s): None.
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Product Information
Description F(ab')2 Anti-RAT IgG (H&L) (GOAT) Antibody (Min X MOUSE Serum Proteins)
Host Goat
Conjugate Unconjugated
Target Species Rat
Reactivity Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Liquid (sterile filtered)
Host Isotype IgG F(ab')2
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Rat IgG was produced by repeated immunization with Rat IgG whole molecule in goat.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
citation

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Background

F(ab')2 Rat IgG (H&L) Antibody (Pre-Adsorbed) was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Rat IgG (H&L) Antibody (Pre-Adsorbed) is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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$ 210.00
Cat# ASR2818
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Availability: 3-5 days
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