F(ab')2 Anti-Goat IgG (H&L) (Phycoerythrin Conjugated) Secondary Antibody
Rabbit Polyclonal, R-Phycoerythrin (RPE)
|Description||F(ab')2 Anti-GOAT IgG [H&L] (RABBIT) Antibody Phycoerythrin conjugated|
|Application ||IF, FC|
|Application Note||IF Microscopy 1:100-1:250|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Goat IgG whole molecule|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||F(ab')2 fragment antibody Anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).|
|Storage Condition||Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Ideal for FLOW Cytometry and Immunofluroescent Microscopy
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