Fab Anti-Goat IgG (H&L) (Peroxidase Conjugated) Secondary Antibody
Donkey Polyclonal, Peroxidase (Horseradish)
|Description||F(ab) Anti-GOAT IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated|
|Application ||WB, E, IC|
|Application Note||ELISA 1:200,000|
Western Blot 1:2,000-1:10,000
|Host Isotype||IgG F(ab)|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Goat IgG (H&L) was produced by repeated immunization with goat whole molecule in donkey.|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Purity||F(ab) Goat IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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