|Description||CHICKEN IgM whole molecule|
|Physical State||Liquid (sterile filtered)|
|Buffer||0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0|
|Species of Origin||Chicken|
|Preservative||0.1% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Chicken IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Chicken IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and anti-Chicken IgM ( µ chain specific). No reaction was observed against anti-Chicken IgG F(c). Some light chain cross reactivity will occur with anti-Chicken IgG. Analysis by SDS-PAGE was used to show purity at greater than 95%. Some high molecular weight banding may be visible. A minor band at ~50 kDa may also be visible.|
|Storage Condition||Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Chicken IgM whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
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