|Description||HUMAN IgM (myeloma) Fc5 µ fragment|
|Physical State||Liquid (sterile filtered)|
|Buffer||0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0|
|Species of Origin||Human|
|Preservative||0.05% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||Human IgM (myeloma) Fc5 µ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. A single precipitin arc was observed against anti-human IgM Fc5 µ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.|
|Storage Condition||Store vial at 4° C prior to opening. Human IgM (myeloma) Fc5 µ fragment is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Human IgM (myeloma) Fc5 µ fragment consists of only the µ (mu) chain of the Fc fragment.
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