Anti-Mouse IgM (H&L) (Peroxidase Conjugated) Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | Anti-MOUSE IgM (H&L) (GOAT) Antibody Peroxidase Conjugated |
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Host | Goat |
Conjugate | Peroxidase (Horseradish) |
Target Species | Mouse |
Clonality | Polyclonal |
Application
| WB, E, IC |
Application Note | ELISA 1:10,000-1:50,000 Western Blot 1:1,000-1:10,000 Immunochemistry 1:500-1:2,500 |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgM (H&L) |
Buffer | 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Anti-Mouse IgM secondary antibody was produced by repeated immunization with Mouse IgM whole molecule in rabbit. |
Reconstitution Volume | 2.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition | Ambient |
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Purity | Anti-Mouse IgM secondary antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Mouse IgM antibody generated in rabbit detects specifically Mouse IgM with some light chain cross reactivity against Mouse IgG. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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