Anti-Mouse IgM (H&L) (Peroxidase Conjugated) Secondary Antibody
Rabbit Polyclonal, Peroxidase (Horseradish)
|Description||Anti-MOUSE IgM (H&L) (RABBIT) Antibody Peroxidase Conjugated|
|Application ||WB, E, IC|
|Application Note||ELISA 1:10,000-1:50,000|
Western Blot 1:1,000-1:10,000
|Target Isotype||IgM (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Mouse IgM (H&L) was produced by repeated immunization with mouse IgM whole molecule in rabbit.|
|Reconstitution Volume||2.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Purity||This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Mouse IgM (H&L) peroxidase conjugated antibody generated in rabbit detects specifically mouse IgM (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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