F(ab')2 Anti-Golden Syrian Hamster IgG (H&L) Secondary Antibody
Rabbit Polyclonal, R-Phycoerythrin (RPE)
|Description||F(ab')2 Anti-GOLDEN SYRIAN HAMSTER IgG (H&L) (RABBIT) Antibody Phycoerythrin conjugated|
|Target Species||Golden Syrian Hamster|
|Application ||IF, FC|
|Application Note||IF Microscopy 1:100-1:250|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Golden Syrian Hamster IgG (H&L) was produced by repeated immunization with Golden Syrian Hamster IgG whole molecule in rabbit.|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||F(ab')2 Anti-Golden Syrian Hamster IgG Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).|
|Storage Condition||Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Golden Syrian Hamster IgG (H&L) Antibody generated in rabbit detects specifically golden syrian hamster IgG. This secondary Anti-Golden Syriam Hamster IgG antibody is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.Anti-Golden Syrian Hamster IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.
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