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>   home   >   Products   >   Secondary Antibodies   >   Texas Red®   >   F(ab')2 Anti-Mouse IgM (mu chain) (Fluorescein Conjugated) Secondary Antibody   

F(ab')2 Anti-Mouse IgM (mu chain) (Fluorescein Conjugated) Secondary Antibody

Goat Polyclonal, Fluorescein (FITC)

     
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Product Information
Description F(ab')2 Anti-MOUSE IgM (mu chain) (GOAT) Antibody Fluorescein Conjugated
Host Goat
Conjugate Fluorescein (FITC)
FP Value 5.4 moles Fluorescein (FITC) per mole of IgG F(ab')2
Target Species Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
FlowCytometry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgM µ chain
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgM mu heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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$ 201.00
Cat# ASR2990
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Availability: 3-5 days
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