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F(ab')2 Anti-Human IgG IgA IgM (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Anti

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

     
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Product Information
Description F(ab')2 Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated Min X MOUSE Serum Proteins
Host Goat
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Human
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunohistochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG F(ab')2
Target Isotype IgG IgA IgM
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Human IgG whole molecule
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum.  This reagent is suitable for the detection of all human immunoglobulins and chain combinations.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or  Mouse Serum Proteins.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

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$ 207.00
Cat# ASR3016
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Availability: 3-5 days
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