Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)
|Description||Anti-CHICKEN IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)|
|Conjugate||Alkaline Phosphatase (Calf Intestine)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:2,000-1:10,000|
Western Blot 1:500-1:2,500
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgG (H&L)|
|Buffer||0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Immunogen||Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat.|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Chicken IgG whole molecule antibody generated in goat detects specifically Chicken IgG whole molecule. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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