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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody   

Anti-Chicken IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

  • WB - Anti-Chicken IgG (H&L)  (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody ASR3083
    Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule . Lane 2: Chicken IgG F(c) Fragment . Lane 3: Chicken IgG F(ab) Fragment . Lane 4: Chicken IgM Whole Molecule . Lane 5: Chicken Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and F(ab), 75 kDa for IgM. Chicken F(c) migrates slightly higher.
  • WB - Anti-Chicken IgG (H&L)  (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody ASR3083
    Western Blot of Alkaline Phosphatase Conjugated Goat anti-Chicken IgG (Pre-Adsorbed) secondary antibody. Lane 1: Chicken IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 72 kDa, 25 & 72 kDa for Chicken IgG. Other band(s): None.
Product Information
Description Anti-CHICKEN IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)
Host Goat
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Chicken
Reactivity Chicken
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat.
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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Anti-Chicken IgG whole molecule antibody generated in goat detects specifically Chicken IgG whole molecule. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 242.00
Cat# ASR3083
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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