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Anti-Mouse IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, R-Phycoerythrin (RPE)

     
  • DB - Anti-Mouse IgG (H&L)  (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody ASR3094
    Dot Blot of Goat anti-Mouse IgG Antibody Phycoerythrin Conjugated. Antigen: Mouse IgG. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Block: 5% BLOTTO 1 Hour at RT.
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Product Information
Description Anti-MOUSE IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Hs Ham Hu Rb Rt &Sh Serum Proteins
Host Goat
Conjugate R-Phycoerythrin (RPE)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
FLOW1:100-1:250
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Background

Anti-Mouse IgG (H&L) Phycoerythrin conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody-based fluorescent assays requiring extremely low background levels.

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$ 243.00
Cat# ASR3094
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