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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (Phycoerythrin Conjugated) Secondary Antibody   

Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (Phycoerythrin Conjugated) Secondary Antibody

Rabbit Polyclonal, R-Phycoerythrin (RPE)

  •  - Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain)  (Phycoerythrin Conjugated) Secondary Antibody ASR3095
    Indirect ELISA of Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, and 3) antibody. Antigen: purified mouse IgG heavy and light chains. Coating amount: 0.1 µg per well. Primary antibody: Rabbit Anti-Mouse IgG (Gamma 1, 2a, 2b, and 3) HRP conjugated Antibody. Dilution series: 2-fold. Substrate: TMB .
Product Information
Description Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody Phycoerythrin Conjugated
Host Rabbit
Conjugate R-Phycoerythrin (RPE)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (gamma 1, 2a, 2b and 3 chain)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgG subclass pan reactive Secondary Antibody was pproduced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Anti-Mouse IgG subclass pan reactive Secondary Antibody Phycoerythrin Conjugated is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays like western blotting.

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$ 242.00
Cat# ASR3095
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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