F(ab')2 Anti-Human IgG (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody
Donkey Polyclonal, Peroxidase (Horseradish)
|Description||F(ab')2 Anti-HUMAN IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins|
|Application ||WB, IHC, E|
|Application Note||ELISA 1:5,000-1:20,000|
Western Blot 1:2,000-1:10,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Human IgG whole molecule|
|Reconstitution Volume||500 µL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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