F(ab')2 Anti-HUMAN IgG F(c) (Pre-adsorbed Phycoerythrin Conjugated) Secondary Antibody
Goat Polyclonal, R-Phycoerythrin (RPE)
|Description||F(ab')2 Anti-HUMAN IgG F(c) (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs Ms & Rt Serum Proteins|
|Application ||IF, FC|
|Application Note||IF Microscopy 1:100-1:250|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG F(c)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat.|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse and Mouse Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
F(ab')2 HUMAN IgG F(c) (Pre-Adsorbed) Phycoerythrin Conjugated Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 HUMAN IgG F(c) (Pre-Adsorbed) Phycoerythrin Conjugated Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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