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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   F(ab')2 Anti-Mouse IgG F(ab')2 (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody   

F(ab')2 Anti-Mouse IgG F(ab')2 (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, R-Phycoerythrin (RPE)

  • WB - F(ab')2  Anti-Mouse IgG F(ab')2  (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody ASR3193
    Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Mouse IgG F(ab’)2 (Pre-Adsorbed) secondary antibody. Lane 1: Mouse F(ab)2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Mouse IgG F(ab’)2. Other band(s): None.
Product Information
Description F(ab')2 Anti-MOUSE IgG F(ab')2 (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs & Hu Serum Proteins
Host Goat
Conjugate R-Phycoerythrin (RPE)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
Western Blot 1:100-1:500
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG F(ab')2
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG F(ab')2 fragment
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity F(ab')2 fragment PE conjugated Anti-Mouse IgG F(ab')2 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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$ 295.00
Cat# ASR3193
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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