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Anti-MOUSE IgG2a (ATTO 488 Conjugated) Pre-adsorbed Secondary Antibody

Goat Polyclonal, ATTO 488

  • IF - Anti-MOUSE IgG2a  (ATTO 488 Conjugated) Pre-adsorbed Secondary Antibody ASR3229
    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
Product Information
Description Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody ATTO 488 Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins)
Host Goat
Conjugate ATTO 488
FP Value 2.7 moles ATTO 488 per mole of IgG
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note FLISA >1:20,000
IF Microscopy >1:5,000
FlowCytometry 1:500-1:2,500
Western Blot 1:4,000-1:20,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG2a
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG2a heavy chain
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Mouse IgG2a secondary antibody is available in a variety of formats. ATTO 488 Conjugated anitbodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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$ 310.00
Cat# ASR3229
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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