Anti-GM-CSF Secondary Antibody
Rabbit Polyclonal, Unconjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | Anti-GM-CSF (RABBIT) Antibody |
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Host | Rabbit |
Conjugate | Unconjugated |
Target Species | Human |
Reactivity | Human |
Clonality | Polyclonal |
Application
| WB, E |
Application Note | ELISA 1:1,000-1:5,000 Western Blot 1:500-1:2,000 |
Physical State | Liquid (sterile filtered) |
Host Isotype | Antiserum |
Buffer | None |
Immunogen | This whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF. |
Stabilizer | None |
Preservative | None |
Shipping Condition | Dry Ice |
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Purity | This antiserum has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. |
Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor; CSF, sargramostim and molgramostin)is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
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