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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Affinity Purified RAT IgG (H&L)(GOAT) (Min X MOUSE Serum Proteins) Secondary Antibody   

Anti-Affinity Purified RAT IgG (H&L)(GOAT) (Min X MOUSE Serum Proteins) Secondary Antibody

Goat Polyclonal, Unconjugated

  • WB - Anti-Affinity Purified RAT IgG (H&L)(GOAT)  (Min X MOUSE Serum Proteins) Secondary Antibody ASR3326
    Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Human Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . Lane 5: Rat Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Human Serum Proteins) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product Information
Description Anti-RAT IgG (H&L) (GOAT) Antibody (Min X Mouse Serum Proteins)
Host Goat
Conjugate Unconjugated
Target Species Rat
Reactivity Rat
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:20,000-1:110,000
Western Blot 1:10,000-1:40,000
Immunochemistry 1:5,000-1:20,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgG whole molecule
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Mouse Serum Proteins.  Specificity was confirmed by ELISA at less than 1% reactivity.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
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$ 1,163.00
Cat# ASR3326
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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