|Description||F(ab')2 Anti-CHICKEN IgG F(ab')2 (RABBIT) Antibody|
|Application ||WB, IHC, E|
|Application Note||ELISA 1:20,000-1:100,000|
Western Blot 1:2,000-1:10,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG F(ab')2|
|Buffer||0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Chicken IgG F(ab')2 fragment|
|Reconstitution Volume||2.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Purity||This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab’)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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