F(ab')2 Anti-Human IgG (H&L) (Fluorescein Conjugated) Secondary Antibody
Goat Polyclonal, Fluorescein (FITC)
|Description||F(ab')2 Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated|
|FP Value||1.1 moles Fluorescein (FITC) per mole of IgG F(ab')2|
|Application ||IF, FC|
|Application Note||FLISA 1:10,000-1:50,000|
IF Microscopy 1:1,000-1:5,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Human IgG whole molecule|
|Reconstitution Volume||2.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Thimerosal|
|Purity||This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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