|Description||Anti-SHEEP IgG (H&L) (DONKEY) Antibody|
|Application ||WB, IHC, E|
|Application Note||ELISA 1:20,000-1:100,000|
Western Blot 1:2,000-1:10,000
|Target Isotype||IgG (H&L)|
|Buffer||0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Sheep IgG whole molecule|
|Reconstitution Volume||5.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||Sheep IgG (H&L) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum. This antibody will detect intact and immunoglobulin components from Sheep.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Sheep IgG unconjugated antibody was generated in donkey and detects specifically sheep IgG. This primary unconjugated anti-Sheep antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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