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Anti-Sheep IgG (H&L) Secondary Antibody
Donkey Polyclonal, Unconjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Description | Anti-SHEEP IgG (H&L) (DONKEY) Antibody |
|---|---|
| Host | Donkey |
| Conjugate | Unconjugated |
| Target Species | Sheep |
| Clonality | Polyclonal |
Application 
| WB, IHC, E |
| Application Note | ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunohistochemistry 1:1,000-1:5,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (H&L) |
| Buffer | 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Sheep IgG whole molecule |
| Reconstitution Volume | 5.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | Sheep IgG (H&L) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum. This antibody will detect intact and immunoglobulin components from Sheep. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Sheep IgG unconjugated antibody was generated in donkey and detects specifically sheep IgG. This primary unconjugated anti-Sheep antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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