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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   MOUSE IgG (BULK ORDER)   


    SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 µg per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa
    SDS-PAGE of Mouse IgG Whole Molecule (BULK ORDER) . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa Myeloma Protein . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
Product Information
Description MOUSE IgG whole molecule (BULK ORDER)
Conjugate Unconjugated
Physical State Lyophilized
Host Isotype IgG
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Mouse
Reconstitution Volume 2.5 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
Storage Condition Store purified Mouse IgG at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains — two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75% of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.

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Cat# ASR3579
(40 western blots)
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