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>   home   >   Products   >   Secondary Antibodies   >   Goat Anti-Mouse IgG2c, Human ads-HRP Secondary Antibody   

Goat Anti-Mouse IgG2c, Human ads-HRP Secondary Antibody

Goat Polyclonal,HRP

Product Information
Description Reacts with the heavy chain of mouse IgG2c as demonstrated by ELISA or FLISA. Minimal cross reactivity with human immunoglobulins.
Cross Adsorption Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins
Host Goat
Clonality Polyclonal
Target Species Mouse
Reactivity Mouse
Isotype IgG
Specificity IgG2c
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
ELISA Dilution 1:4,000 – 1:8,000
Antigen Description Recently it became evident that inbred mouse strains with the Igh-1b allele do not have the gene for IgG2a and instead express the IgG2c isotype.1 Commercial anti-IgG2a antibodies cross react inadequately with IgG2c in ELISA and immunoblot and hence, may not be suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD.2, 3 SouthernBiotech has developed a new IgG2cspecific antibody for accurate detection and quantitation of this isotype in mouse strains with the Igh-1b allele.
Additional Information
Source Pooled antisera from goats hyperimmunized with C57BL/6 IgG2c proteins
Purification Affinity chromatography on mouse IgG2c covalently linked to agarose
Storage Condition The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of (PBS/NaN3) as preservative. Store at 2- 8°C. Reagents are stable for the period shown on the label if stored as directed.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Citations (0)

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1. Martin, R.M., J.L. Brady, and A.L. Lew. 1998. J. Immunol. Meth. 212:187.
2. Parsons, M., V.T. Oi, C.M. Huang, and L. A. Herzenberg. 1983. Immunogenetics 18:323.
3. Morgado, M.G., P. Cam, C.Gris-Liebe, P.A. Cazenave, and E.Jouvin-Marche. 1989. EMBO J. 8:3245.
4. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, et al. Batf coordinates multiple aspects of B and T cell function required for
normal antibody responses. J. Exp. Med. 2010: 207(5); 933-942.

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$ 132.00
Cat# ASS1030
Availability: 3-5 days
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