|Description||Reacts with the heavy chain of mouse IgG2c as demonstrated by ELISA or FLISA. Minimal cross reactivity with human immunoglobulins.|
|Cross Adsorption||Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins|
|Application ||WB, IHC, E|
|ELISA Dilution||1:4,000 – 1:8,000|
|Antigen Description||Recently it became evident that inbred mouse strains with the Igh-1b allele do not have the gene for IgG2a and instead express the IgG2c isotype.1 Commercial anti-IgG2a antibodies cross react inadequately with IgG2c in ELISA and immunoblot and hence, may not be suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD.2, 3 SouthernBiotech has developed a new IgG2cspecific antibody for accurate detection and quantitation of this isotype in mouse strains with the Igh-1b allele.|
|Source||Pooled antisera from goats hyperimmunized with C57BL/6 IgG2c proteins|
|Purification||Affinity chromatography on mouse IgG2c covalently linked to agarose|
|Storage Condition||The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of (PBS/NaN3) as preservative. Store at 2- 8°C. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, R.M., J.L. Brady, and A.L. Lew. 1998. J. Immunol. Meth. 212:187.
2. Parsons, M., V.T. Oi, C.M. Huang, and L. A. Herzenberg. 1983. Immunogenetics 18:323.
3. Morgado, M.G., P. Cam, C.Gris-Liebe, P.A. Cazenave, and E.Jouvin-Marche. 1989. EMBO J. 8:3245.
4. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, et al. Batf coordinates multiple aspects of B and T cell function required for
normal antibody responses. J. Exp. Med. 2010: 207(5); 933-942.
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