|Description||Reacts with the heavy chain of mouse IgM as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins.|
|Cross Adsorption||Mouse IgG1, IgG2a, IgG2b, IgG3, and IgA, pooled human sera and purified human paraproteins|
|Application ||WB, IHC, E|
|ELISA Dilution||1:4,000 – 1:8,000|
|Source||Pooled antisera from goats hyperimmunized with mouse IgM paraproteins|
|Purification||Affinity chromatography on mouse IgM covalently linked to agarose|
|Storage Condition||The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD), Cyanine 5 (CY5), Alexa Fluor® 488 (AF488), Alexa Fluor® 647 (AF647) and Alexa Fluor® 555 (AF555) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at -20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The β-galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8°C. The R-phycoerythrin (PE) and allophycocyanin (APC) conjugates are supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! The R-phycoerythrin-Cyanine 7 (PE/CY7) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
1. Brouwer E, Huitema MG, Mulder AHL, Heeringa P, van Goor H, Tervaert JWC, et al. Neutrophil activation in vitro and in vivo in Wegener’s
granulomatosis. Kidney Int. 1994; 45: 1120-1131.
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