|Description||Reacts with the heavy chain of mouse IgG2c as demonstrated by ELISA or FLISA. Minimal cross reactivity with human immunoglobulins.|
|Cross Adsorption||Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins|
|Application ||WB, IHC, E|
|ELISA Dilution||1:2,000 – 1:4,000|
|Antigen Description||Recently it became evident that inbred mouse strains with the Igh-1b allele do not have the gene for IgG2a and instead express the IgG2c isotype.1 Commercial anti-IgG2a antibodies cross react inadequately with IgG2c in ELISA and immunoblot and hence, may not be suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD.2, 3 SouthernBiotech has developed a new IgG2cspecific antibody for accurate detection and quantitation of this isotype in mouse strains with the Igh-1b allele.|
|Source||Pooled antisera from goats hyperimmunized with C57BL/6 IgG2c proteins|
|Purification||Affinity chromatography on mouse IgG2c covalently linked to agarose|
|Storage Condition||The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of (PBS/NaN3) as preservative. Store at 2- 8°C. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Martin, R.M., J.L. Brady, and A.L. Lew. 1998. J. Immunol. Meth. 212:187.
2. Parsons, M., V.T. Oi, C.M. Huang, and L. A. Herzenberg. 1983. Immunogenetics 18:323.
3. Morgado, M.G., P. Cam, C.Gris-Liebe, P.A. Cazenave, and E.Jouvin-Marche. 1989. EMBO J. 8:3245.
4. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, et al. Batf coordinates multiple aspects of B and T cell function required for
normal antibody responses. J. Exp. Med. 2010: 207(5); 933-942.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.