|Description||Reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b IgG3, and IgA as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.|
|Cross Adsorption||Pooled human sera and purified human paraproteins|
|Application ||WB, IHC, E|
|ELISA Dilution||1:2,000 – 1:4,000|
|Source||Pepsin digest of goat anti-mouse Ig|
|Purification||Gel filtration chromatography of pepsin digested antibody|
|Storage Condition||The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC), Cyanine 5 (CY5), Alexa Fluor® 488 (AF488), Alexa Fluor® 555 (AF555), and Alexa Fluor® 647 (AF647) conjugates are supplied conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The R-phycoerythrin (PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! The Spectral Red® (SPRD), R-phycoerythrin-Texas Red (PE/TXRD), and R-phycoerythrin-Cyanine 5.5 (PE/CY5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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