|Description||Reacts with the µ heavy chain of mouse IgM as demonstrated by ELISA|
|Application ||WB, IHC, E|
|ELISA Dilution||1:4,000 – 1:8,000|
|Storage Condition||The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C The R-phycoerythrin (PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! The R-phycoerythrin-Cyanine 7 (PE/CY7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Dovat, S, Montecina-Rodriguez E, Schuman V, Teitell MA, Dorshkind K, and Smale ST Transgenic Expression of Helios in B Lineage
Cells Alters B Cell Properties and Promotes Lymphomagenesis . J Immunol 2005; 175(6): 3508-3515.
2. Berretta F, St-Pierre J, Piccirillo CA, and Stevenson MM IL-2 Contributes to Maintaining a Balance between CD4+Foxp3+ Regulatory T
Cells and Effector CD4+ T Cells Required for Immune Control of Blood-Stage Malaria Infection. J Immunol 2001; 186(8): 4862-4871.
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