|Description||Reacts with the µ heavy chain of mouse IgM as demonstrated by ELISA|
|Application ||WB, IHC, E|
|ELISA Dilution||1:1,000 – 1:4,000|
|Storage Condition||The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C The R-phycoerythrin (PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! The R-phycoerythrin-Cyanine 7 (PE/CY7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
1. Dovat, S, Montecina-Rodriguez E, Schuman V, Teitell MA, Dorshkind K, and Smale ST Transgenic Expression of Helios in B Lineage
Cells Alters B Cell Properties and Promotes Lymphomagenesis . J Immunol 2005; 175(6): 3508-3515.
2. Berretta F, St-Pierre J, Piccirillo CA, and Stevenson MM IL-2 Contributes to Maintaining a Balance between CD4+Foxp3+ Regulatory T
Cells and Effector CD4+ T Cells Required for Immune Control of Blood-Stage Malaria Infection. J Immunol 2001; 186(8): 4862-4871.
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