|Description||Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA and flow cytometry; may react with other species, minimal cross reactivity with human immunoglobulins|
|Cross Adsorption||Pooled human sera and purified human paraproteins|
|Application ||WB, IHC, E|
|ELISA Dilution||1:2,000 – 1:4,000|
|Source||Pepsin digest of rabbit anti-mouse IgG(H+L)|
|Purification||Affinity chromatography on mouse IgG covalently coupled to agarose gel filtration chromatography of pepsin digested antibody|
|Storage Condition||The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8°C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8°C or long-term at - 20°C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.