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AADAC Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant AADAC.

     
  • WB - AADAC Antibody (monoclonal) (M01) AT1002a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • E - AADAC Antibody (monoclonal) (M01) AT1002a
    Detection limit for recombinant GST tagged AADAC is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P22760
Other Accession NM_001086
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 2E9
Calculated MW 45734 Da
Additional Information
Gene ID 13
Other Names Arylacetamide deacetylase, AADAC, DAC
Target/Specificity AADAC (NP_001077, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAADAC Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens

References

1.N-Glycosylation during translation is essential for human arylacetamide deacetylase enzyme activity.Muta K, Fukami T, Nakajima M, Yokoi TBiochem Pharmacol. 2013 Oct 11. pii: S0006-2952(13)00651-5. doi: 10.1016/j.bcp.2013.10.001.2.Metabolic activation by human arylacetamide deacetylase, CYP2E1, and CYP1A2 causes phenacetin-induced methemoglobinemia.Kobayashi Y, Fukami T, Higuchi R, Nakajima M, Yokoi T.Biochem Pharmacol. 2012 Aug 23.3.A Novel Polymorphic Allele of Human Arylacetamide Deacetylase Leads to Diminished Enzyme Activity.Shimizu M, Fukami T, Kobayashi Y, Takamiya M, Aoki Y, Nakajima M, Yokoi T.Drug Metab Dispos. 2012 Mar 13. [Epub ahead of print]4.Species differences in tissue distribution and enzyme activities of arylacetamide deacetylase in human, rat, and mouse.Kobayashi Y, Fukami T, Nakajima A, Watanabe A, Nakajima M, Yokoi T.Drug Metab Dispos. 2012 Apr;40(4):671-9. Epub 2011 Dec 29.5.Human Arylacetamide Deacetylase Is A Principal Enzyme in Flutamide Hydrolysis.Watanabe A, Fukami T, Nakajima M, Takamiya M, Aoki Y, Yokoi T.Drug Metab Dispos. 2009 Jul;37(7):1513-20. Epub 2009 Apr 1.

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$ 350.00
Cat# AT1002a
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Availability: 7-10 days
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