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ACTN1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ACTN1.

  • WB - ACTN1 Antibody (monoclonal) (M01) AT1032a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.41 KDa) .
  • WB - ACTN1 Antibody (monoclonal) (M01) AT1032a
    Western Blot analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.

    Lane 1: ACTN1 transfected lysate(103.1 KDa).
    Lane 2: Non-transfected lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12814
Other Accession NM_001102
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 3F1
Calculated MW 103058 Da
Additional Information
Gene ID 87
Other Names Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1
Target/Specificity ACTN1 (NP_001093, 543 a.a. ~ 639 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsACTN1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.


1.Rab13 Small G Protein and Junctional Rab13-binding Protein (JRAB) Orchestrate Actin Cytoskeletal Organization during Epithelial Junctional Development.Sakane A, Abdallah AA, Nakano K, Honda K, Ikeda W, Nishikawa Y, Matsumoto M, Matsushita N, Kitamura T, Sasaki T.J Biol Chem. 2012 Dec 14;287(51):42455-68. doi: 10.1074/jbc.M112.383653. Epub 2012 Oct 24.2.alpha-actinin 1 and alpha-actinin 4: Contrasting roles in the survival, motility, and rhoa signaling of astrocytoma cells.Quick Q, Skalli O.Exp Cell Res. 2010 Feb 12. [Epub ahead of print]

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$ 315.00
Cat# AT1032a
(40 western blots)
Availability: 7-10 days
Bulk Size
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