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AKAP12 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant AKAP12.

  • WB - AKAP12 Antibody (monoclonal) (M01) AT1083a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.62 KDa) .
  • E - AKAP12 Antibody (monoclonal) (M01) AT1083a
    Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q02952
Other Accession NM_005100
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 1C5
Calculated MW 191482 Da
Additional Information
Gene ID 9590
Other Names A-kinase anchor protein 12, AKAP-12, A-kinase anchor protein 250 kDa, AKAP 250, Gravin, Myasthenia gravis autoantigen, AKAP12, AKAP250
Target/Specificity AKAP12 (NP_005091, 1675 a.a. ~ 1782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAKAP12 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.


1.iTRAQ analysis of colorectal cancer cell lines suggests Drebrin (DBN1) is overexpressed during liver metastasis.Lin Q, Tan HT, Lim TK, Khoo A, Lim KH, Chung MCProteomics. 2014 Jun;14(11):1434-43. doi: 10.1002/pmic.201300462. Epub 2014 Apr 17.2.Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells.Tan HT, Tan S, Lin Q, Lim TK, Hew CL, Chung MC.Mol Cell Proteomics. 2008 Jun;7(6):1174-85. Epub 2008 Mar 14.

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$ 315.00
Cat# AT1083a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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