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ALAS1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ALAS1.

     
  • WB - ALAS1 Antibody (monoclonal) (M01) AT1105a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
    detail
  • WB - ALAS1 Antibody (monoclonal) (M01) AT1105a
    ALAS1 monoclonal antibody (M01), clone 3G10 Western Blot analysis of ALAS1 expression in JAR ( (Cat # AT1105a )
    detail
  • E - ALAS1 Antibody (monoclonal) (M01) AT1105a
    Detection limit for recombinant GST tagged ALAS1 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P13196
Other Accession NM_000688
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3G10
Calculated MW 70581 Da
Additional Information
Gene ID 211
Other Names 5-aminolevulinate synthase, nonspecific, mitochondrial, ALAS-H, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, Delta-aminolevulinate synthase 1, ALAS1, ALAS3, ALASH
Target/Specificity ALAS1 (NP_000679, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsALAS1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).

References

1.PGC-1{alpha} is not mandatory for exercise- and training-induced adaptive gene responsens in mouse skeletal muscle.Leick L, Wojtaszewski JF, Johansen ST, Kiilerich K, Comes G, Hellsten Y, Hidalgo J, Pilegaard H.Am J Physiol Endocrinol Metab. 2008 Feb;294(2):E463-74. Epub 2007 Dec 11.

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$ 350.00
Cat# AT1105a
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Availability: 7-10 days
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