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>   home   >   Products   >   Primary Antibodies   >   ALF Antibody (monoclonal) (M05)   

ALF Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant ALF.

  • WB - ALF Antibody (monoclonal) (M05) AT1119a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
  • WB - ALF Antibody (monoclonal) (M05) AT1119a
    Western Blot analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4.

    Lane 1: ALF transfected lysate(52.4 KDa).
    Lane 2: Non-transfected lysate.
  • IF - ALF Antibody (monoclonal) (M05) AT1119a
    Immunofluorescence of monoclonal antibody to GTF2A1L on HeLa cell . [antibody concentration 10 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UNN4
Other Accession NM_006872
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3E5
Calculated MW 52444 Da
Additional Information
Gene ID 11036
Other Names TFIIA-alpha and beta-like factor, General transcription factor II A, 1-like factor, GTF2A1L, ALF, GTF2A1LF
Target/Specificity ALF (NP_006863, 251 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsALF Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene. Kim M, et al. J Biol Chem, 2006 Nov 10. PMID 16966320.Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo. Howe ML, et al. Stem Cells Dev, 2006 Apr. PMID 16646664.Involvement of ALF in human spermatogenesis and male infertility. Huang M, et al. Int J Mol Med, 2006 Apr. PMID 16525715.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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$ 315.00
Cat# AT1119a
(40 western blots)
Availability: 7-10 days
Bulk Size
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