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AMY1A Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant AMY1A.

     
  • WB - AMY1A Antibody (monoclonal) (M04) AT1135a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33.88 KDa) .
    detail
  • WB - AMY1A Antibody (monoclonal) (M04) AT1135a
    AMY1A monoclonal antibody (M04), clone 2D4. Western Blot analysis of AMY1A expression in human pancreas.
    detail
  • IHC - AMY1A Antibody (monoclonal) (M04) AT1135a
    Immunoperoxidase of monoclonal antibody to AMY1A on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P04745
Other Accession NM_001008221
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2D4
Additional Information
Other Names Alpha-amylase 1, 4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase, AMY1A, AMY1
Target/Specificity AMY1A (NP_001008222, 172 a.a. ~ 245 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAMY1A Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.

References

Probing the role of aromatic residues at the secondary saccharide-binding sites of human salivary alpha-amylase in substrate hydrolysis and bacterial binding. Ragunath C, et al. J Mol Biol, 2008 Dec 31. PMID 18951906.Social evaluation-induced amylase elevation and economic decision-making in the dictator game in humans. Takahashi T, et al. Neuro Endocrinol Lett, 2007 Oct. PMID 17984929.Diet and the evolution of human amylase gene copy number variation. Perry GH, et al. Nat Genet, 2007 Oct. PMID 17828263.Interaction of salivary alpha-amylase and amylase-binding-protein A (AbpA) of Streptococcus gordonii with glucosyltransferase of S. gordonii and Streptococcus mutans. Chaudhuri B, et al. BMC Microbiol, 2007 Jun 25. PMID 17593303.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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$ 350.00
Cat# AT1135a
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Availability: 7-10 days
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