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AP2S1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant AP2S1.

  • WB - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (41.36 KDa) .
  • WB - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Western Blot analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.

    Lane 1: AP2S1 transfected lysate(17 KDa).
    Lane 2: Non-transfected lysate.
  • E - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/ml as a capture antibody.
  • WB - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Western blot analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ( (Cat # AT1153a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P53680
Other Accession BC006337
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3E5
Calculated MW 17018 Da
Additional Information
Gene ID 1175
Other Names AP-2 complex subunit sigma, Adaptor protein complex AP-2 subunit sigma, Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 sigma small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, HA2 17 kDa subunit, Plasma membrane adaptor AP-2 17 kDa protein, Sigma2-adaptin, AP2S1, AP17, CLAPS2
Target/Specificity AP2S1 (AAH06337, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAP2S1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts.


Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.A conserved dileucine motif mediates clathrin and AP-2-dependent endocytosis of the HIV-1 envelope protein. Byland R, et al. Mol Biol Cell, 2007 Feb. PMID 17108326.Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2. Batonick M, et al. Virology, 2005 Nov 25. PMID 16139856.Human immunodeficiency virus Nef induces rapid internalization of the T-cell coreceptor CD8alphabeta. Stove V, et al. J Virol, 2005 Sep. PMID 16103193.PICK1 interacts with ABP/GRIP to regulate AMPA receptor trafficking. Lu W, et al. Neuron, 2005 Aug 4. PMID 16055064.

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$ 315.00
Cat# AT1153a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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