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ARID1A monoclonal antibody (M01A)

Purified Mouse Monoclonal Antibody (Mab)

  • WB - ARID1A monoclonal antibody (M01A) AT1188a
    Western Blot detection against Immunogen (37.84 KDa) .
  •  - ARID1A monoclonal antibody (M01A) AT1188a
    Detection limit for recombinant GST tagged ARID1A is 0.3 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14497
Other Accession o14497, NP_006006
Reactivity Human
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3H2
Calculated MW 242045 Da
Additional Information
Gene ID 8289
Other Names AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BAF250, BRG1-associated factor 250a, BAF250A, Osa homolog 1, hOSA1, SWI-like protein, SWI/SNF complex protein p270, SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1, hELD, ARID1A, BAF250, BAF250A, C1orf4, OSA1, SMARCF1
Target/Specificity Mouse monoclonal antibody raised against a partial recombinant ARID1A. ARID1A (NP_006006, 1216 a.a. ~ 1325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsARID1A monoclonal antibody (M01A) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )


This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.


Mammalian SWI/SNF--a subunit BAF250/ARID1 is an E3 ubiquitin ligase that targets histone H2B. Li XS, et al. Mol Cell Biol, 2010 Apr. PMID 20086098. HIC1 interacts with a specific subunit of SWI/SNF complexes, ARID1A/BAF250A. Van Rechem C, et al. Biochem Biophys Res Commun, 2009 Aug 7. PMID 19486893. Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348. Genomic and functional evidence for an ARID1A tumor suppressor role. Huang J, et al. Genes Chromosomes Cancer, 2007 Aug. PMID 17492758. Distinct mammalian SWI/SNF chromatin remodeling complexes with opposing roles in cell-cycle control. Nagl NG Jr, et al. EMBO J, 2007 Feb 7. PMID 17255939.

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Cat# AT1188a
(40 western blots)
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