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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ARNT Antibody (monoclonal) (M01)   

ARNT Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ARNT.

  • WB - ARNT Antibody (monoclonal) (M01) AT1199a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - ARNT Antibody (monoclonal) (M01) AT1199a
    ARNT monoclonal antibody (M01), clone 3D10 Western Blot analysis of ARNT expression in Hela S3 NE ( (Cat # AT1199a )
  • WB - ARNT Antibody (monoclonal) (M01) AT1199a
    Western Blot analysis of ARNT expression in transfected 293T cell line by ARNT monoclonal antibody (M01), clone 3D10.

    Lane 1: ARNT transfected lysate(86.6 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - ARNT Antibody (monoclonal) (M01) AT1199a
    Immunoperoxidase of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
  • IF - ARNT Antibody (monoclonal) (M01) AT1199a
    Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10 ug/ml]
  • E - ARNT Antibody (monoclonal) (M01) AT1199a
    Detection limit for recombinant GST tagged ARNT is approximately 0.03ng/ml as a capture antibody.
  • WB - ARNT Antibody (monoclonal) (M01) AT1199a
    Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi ( (Cat # AT1199a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27540
Other Accession BC060838
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3D10
Calculated MW 86636 Da
Additional Information
Gene ID 405
Other Names Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2
Target/Specificity ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsARNT Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.


Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia. Jugessur A, et al. PLoS One, 2010 Jul 9. PMID 20634891.Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.MicroRNA-101 negatively regulates Ezh2 and its expression is modulated by androgen receptor and HIF-1alpha/HIF-1beta. Cao P, et al. Mol Cancer, 2010 May 17. PMID 20478051.A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.

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$ 315.00
Cat# AT1199a
(40 western blots)
Availability: 7-10 days
Bulk Size
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