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ASAHL Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NAAA.

     
  • IF - ASAHL Antibody (monoclonal) (M01) AT1208a
    Immunofluorescence of monoclonal antibody to NAAA on NIH/3T3 cell. [antibody concentration 10 ug/ml]
    detail
  • WB - ASAHL Antibody (monoclonal) (M01) AT1208a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33.33 KDa) .
    detail
  • WB - ASAHL Antibody (monoclonal) (M01) AT1208a
    NAAA monoclonal antibody (M01), clone 5E3 Western Blot analysis of ASAHL expression in NIH/3T3 ( (Cat # AT1208a )
    detail
  • E - ASAHL Antibody (monoclonal) (M01) AT1208a
    Detection limit for recombinant GST tagged NAAA is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q02083
Other Accession NM_014435
Reactivity Human, Mouse
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5E4
Calculated MW 40066 Da
Additional Information
Gene ID 27163
Other Names N-acylethanolamine-hydrolyzing acid amidase, 351-, Acid ceramidase-like protein, N-acylsphingosine amidohydrolase-like, ASAH-like protein, N-acylethanolamine-hydrolyzing acid amidase subunit alpha, N-acylethanolamine-hydrolyzing acid amidase subunit beta, NAAA, ASAHL, PLT
Target/Specificity NAAA (NP_055250, 36 a.a. ~ 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsASAHL Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene.

References

Lack of association of genetic variants in genes of the endocannabinoid system with anorexia nervosa. M?ller TD, et al. Child Adolesc Psychiatry Ment Health, 2008 Nov 17. PMID 19014633.Expression and secretion of N-acylethanolamine-hydrolysing acid amidase in human prostate cancer cells. Wang J, et al. J Biochem, 2008 Nov. PMID 18806270.Overexpression and mass spectrometry analysis of mature human acid ceramidase. Schulze H, et al. Biol Chem, 2007 Dec. PMID 18020949.Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism. Zhao LY, et al. Biochim Biophys Acta, 2007 Nov. PMID 17980170.Transcriptome analysis of human gastric cancer. Oh JH, et al. Mamm Genome, 2005 Dec. PMID 16341674.

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$ 350.00
Cat# AT1208a
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Availability: 7-10 days
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