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ATF3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ATF3.

  • WB - ATF3 Antibody (monoclonal) (M01) AT1222a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (45.65 KDa) .
  • WB - ATF3 Antibody (monoclonal) (M01) AT1222a
    Western Blot analysis of ATF3 expression in transfected 293T cell line by ATF3 monoclonal antibody (M01), clone 6B8.

    Lane 1: ATF3 transfected lysate(20.6 KDa).
    Lane 2: Non-transfected lysate.
  • E - ATF3 Antibody (monoclonal) (M01) AT1222a
    Detection limit for recombinant GST tagged ATF3 is approximately 1ng/ml as a capture antibody.
  • WB - ATF3 Antibody (monoclonal) (M01) AT1222a
    Western blot analysis of ATF3 over-expressed 293 cell line, cotransfected with ATF3 Validated Chimera RNAi ( (Cat # AT1222a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18847
Other Accession BC006322
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 6B8
Calculated MW 20576 Da
Additional Information
Gene ID 467
Other Names Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3
Target/Specificity ATF3 (AAH06322, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsATF3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.


1.Transcriptional regulators of the ?GNp63: Their role in limbal epithelial cell proliferation.Hsueh YJ, Kuo PC, Chen JK.J Cell Physiol. 2012 Jul 17. doi: 10.1002/jcp.24160.2.Steroids and extracellular signal-regulated kinase 1/2 activity suppress activating transcription factor 3 expression in patients with severe asthma.Roussel L, Robins S, Schachter A, Berube J, Hamid Q, Rousseau S.J Allergy Clin Immunol. 2011 Apr 22. [Epub ahead of print]3.Energy restriction-mimetic agents induce apoptosis in prostate cancer cells, in part, through epigenetic activation of KLF6 tumor suppressor gene expression.Chen CH, Huang PH, Chu PC, Chen MC, Chou CC, Wang D, Kulp SK, Teng CM, Wang Q, Chen CS.J Biol Chem. 2011 Feb 3. [Epub ahead of print]

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$ 315.00
Cat# AT1222a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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