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>   home   >   Products   >   Primary Antibodies   >   ATF4 Antibody (monoclonal) (M01)   

ATF4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ATF4.

  • WB - ATF4 Antibody (monoclonal) (M01) AT1224a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - ATF4 Antibody (monoclonal) (M01) AT1224a
    Western Blot analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.

    Lane 1: ATF4 transfected lysate(38.6 KDa).
    Lane 2: Non-transfected lysate.
  • IF - ATF4 Antibody (monoclonal) (M01) AT1224a
    Immunofluorescence of monoclonal antibody to ATF4 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - ATF4 Antibody (monoclonal) (M01) AT1224a
    Detection limit for recombinant GST tagged ATF4 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18848
Other Accession NM_001675
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2B3
Calculated MW 38590 Da
Additional Information
Gene ID 468
Other Names Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, DNA-binding protein TAXREB67, Tax-responsive enhancer element-binding protein 67, TaxREB67, ATF4, CREB2, TXREB
Target/Specificity ATF4 (NP_001666.2, 171 a.a. ~ 270 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsATF4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.


1.A Protective Role of Heme-Regulated eIF2α kinase in Cadmium-Induced Toxicity in Erythroid Cells.Wang L, Wang X, Zhang S, Qu G, Liu SFood Chem Toxicol. 2013 Oct 22;62C:880-891. doi: 10.1016/j.fct.2013.10.017.2.Decreased vitamin B12 availability induces ER stress through impaired SIRT1-deacetylation of HSF1.Ghemrawi R, Pooya S, Lorentz S, Gauchotte G, Arnold C, Gueant JL, Battaglia-Hsu SFCell Death Dis. 2013 Mar 21;4:e553. doi: 10.1038/cddis.2013.69.3.Heme-regulated eIF2? kinase activated Atf4 signaling pathway in oxidative stress and erythropoiesis.Suragani RN, Zachariah RS, Velazquez JG, Liu S, Sun CW, Townes TM, Chen JJ.Blood. 2012 May 31;119(22):5276-84. Epub 2012 Apr 12.4.12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response.Cole BK, Kuhn NS, Green-Mitchell SM, Leone KA, Raab RM, Nadler JL, Chakrabarti SK.Am J Physiol Endocrinol Metab. 2012 Mar;302(6):E654-65. Epub 2012 Jan 3.5.Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells.Meister S, Frey B, Lang VR, Gaipl US, Schett G, Schlotzer-Schrehardt U, Voll RE.Neoplasia. 2010 Jul;12(7):550-61.

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$ 315.00
Cat# AT1224a
(40 western blots)
Availability: 7-10 days
Bulk Size
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