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>   home   >   Products   >   Primary Antibodies   >   ATP6AP1 Antibody (monoclonal) (M01)   

ATP6AP1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ATP6AP1.

     
  • WB - ATP6AP1 Antibody (monoclonal) (M01) AT1239a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - ATP6AP1 Antibody (monoclonal) (M01) AT1239a
    ATP6AP1 monoclonal antibody (M01), clone 3A2 Western Blot analysis of ATP6AP1 expression in HeLa ( (Cat # AT1239a )
    detail
  • WB - ATP6AP1 Antibody (monoclonal) (M01) AT1239a
    ATP6AP1 monoclonal antibody (M01), clone 3A2. Western Blot analysis of ATP6AP1 expression in PC-12 ( (Cat # AT1239a )
    detail
  • WB - ATP6AP1 Antibody (monoclonal) (M01) AT1239a
    Western Blot analysis of ATP6AP1 expression in transfected 293T cell line by ATP6AP1 monoclonal antibody (M01), clone 3A2.

    Lane 1: ATP6AP1 transfected lysate(52 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - ATP6AP1 Antibody (monoclonal) (M01) AT1239a
    Detection limit for recombinant GST tagged ATP6AP1 is 0.1 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15904
Other Accession NM_001183
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3A2
Calculated MW 52026 Da
Additional Information
Gene ID 537
Other Names V-type proton ATPase subunit S1, V-ATPase subunit S1, Protein XAP-3, V-ATPase Ac45 subunit, V-ATPase S1 accessory protein, Vacuolar proton pump subunit S1, ATP6AP1, ATP6IP1, ATP6S1, VATPS1, XAP3
Target/Specificity ATP6AP1 (NP_001174, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsATP6AP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
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Background

This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.

References

1.Vacuolar H+-ATPase subunit Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake and storage.Saw NM, Kang SY, Parsaud L, Han GA, Jiang T, Grzegorczyk K, Surkont M, Sun-Wada GH, Wada Y, Li L, Sugita S.Mol Biol Cell. 2011 Jul 27. [Epub ahead of print]

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$ 350.00
Cat# AT1239a
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