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ATP6V1B2 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant ATP6V1B2.

  • E - ATP6V1B2 Antibody (monoclonal) (M02) AT1241a
    Detection limit for recombinant GST tagged ATP6V1B2 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21281
Other Accession BC003100
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names M1
Calculated MW 56501 Da
Additional Information
Gene ID 526
Other Names V-type proton ATPase subunit B, brain isoform, V-ATPase subunit B 2, Endomembrane proton pump 58 kDa subunit, HO57, Vacuolar proton pump subunit B 2, ATP6V1B2, ATP6B2, VPP3
Target/Specificity ATP6V1B2 (AAH03100, 1 a.a. ~ 511 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsATP6V1B2 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.


1.The histone deacetylase inhibitor trichostatin A reduces lysosomal pH and enhances cisplatin-induced apoptosis.Eriksson I, Joosten M, Roberg K, Ollinger K.Exp Cell Res. 2013 Jan 1;319(1):12-20. doi: 10.1016/ j.yexcr.2012.10.004. Epub 2012 Oct 12.2.Intrinsic differences in cisplatin sensitivity of head and neck cancer cell lines: Correlation to lysosomal pH.Nilsson C, Roberg K, Grafstrom RC, Ollinger K.Head Neck. 2010 Sep;32(9):1185-94.

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$ 315.00
Cat# AT1241a
(40 western blots)
Availability: 7-10 days
Bulk Size
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